abstract：:The int gene of phage lambda encodes a protein involved in site-specific recombination. Its expression is regulated differentially during successive phases of the lambda infective cycle. The gene is transcribed early after infection from one promoter, pL, and later from a second promoter pI. Each transcription event r...

journal_title：Journal of molecular biology

authors： Schmeissner U,McKenney K,Rosenberg M,Court D

更新日期：1984-06-15 00:00:00

abstract：:We have studied the reversible dissociation of core size DNA from chicken erythrocyte nucleosome core particles in solutions containing 0 X 1 M to 0 X 6 M-NaCl. Dissociation increases with increasing NaCl concentration, increasing temperature and decreasing particle concentration. At high particle concentrations, no f...

journal_title：Journal of molecular biology

authors： Ausio J,Seger D,Eisenberg H

更新日期：1984-06-15 00:00:00

abstract：:The C repeat family was first observed in the rabbit beta-like gene family. We have estimated the repetition frequency of the C repeats, determined the nucleotide sequence of three intact members and one truncated member, and have investigated the size, tissue specificity, and intracellular localization of C repeat tr...

journal_title：Journal of molecular biology

authors： Cheng JF,Printz R,Callaghan T,Shuey D,Hardison RC

更新日期：1984-06-15 00:00:00

abstract：:We present the DNA sequence alterations due to seven lamB missense mutations yielding resistance to phages lambda and K10. They reveal five different amino acid positions in the LamB protein. Three positions (245, 247 and 249) define a new region required for phage adsorption. The two other positions (148 and 152) bel...

journal_title：Journal of molecular biology

authors： Charbit A,Clement JM,Hofnung M

更新日期：1984-05-25 00:00:00

abstract：:The entire DNA of Rhizobium meliloti MVII /1 cells was isolated preparatively by gentle lysis and sucrose gradient centrifugation. Fractions of the sucrose gradient were investigated by electron microscopy. We found intact megaplasmids in supercoiled and relaxed form and total chromosomes. The length of the megaplasmi...

journal_title：Journal of molecular biology

authors： Burkardt B,Burkardt HJ

更新日期：1984-05-15 00:00:00

abstract：:A survey of known protein structures reveals that approximately 70% of serine residues and at least 85% (potentially 100%) of threonine residues in helices make hydrogen bonds to carbonyl oxygen atoms in the preceding turn of the helix. The high frequency of intrahelical hydrogen bonding is of particular significance ...

journal_title：Journal of molecular biology

authors： Gray TM,Matthews BW

更新日期：1984-05-05 00:00:00

abstract：:Crystals of a relatively unspecific NAD(P)H dehydrogenase from human erythrocytes suitable for X-ray analysis have been grown. They belong to space group P4(1)2(1)2 or its enantiomorph P4(3)2(1)2 with unit cell dimensions: a = b = 79.3 A and c = 38.1 A. The asymmetric unit contains one molecule of Mr 18,000. ...

journal_title：Journal of molecular biology

authors： Pai EF

更新日期：1984-04-25 00:00:00

abstract：:The steps of UUC recognition by tRNAPhe were analysed by temperature-jump measurements. At ion concentrations close to physiological conditions we found three relaxation processes, which we assigned to (1) formation of codon-anticodon complexes, (2) a conformational change of the anticodon loop coupled with Mg2+ bindi...

journal_title：Journal of molecular biology

authors： Labuda D,Striker G,Porschke D

更新日期：1984-04-25 00:00:00

abstract：:A map of the 30 S ribosomal subunit is presented giving the positions of 15 of its 21 proteins. The components located in the map are S1, S3, S4, S5, S6, S7, S8, S9, S10, S11, S12, S14, S15, S18 and S20. ...

journal_title：Journal of molecular biology

authors： Ramakrishnan V,Capel M,Kjeldgaard M,Engelman DM,Moore PB

更新日期：1984-04-05 00:00:00

abstract：:The movement of the bacterial insertion sequence IS1 often generates cointegrate structures in which donor and target replicons are connected by direct repeats of IS1. The experiments reported here were designed to understand how IS1 transposition is controlled. Our physical characterization of the structures of coint...

journal_title：Journal of molecular biology

authors： Biel SW,Adelt G,Berg DE

更新日期：1984-04-05 00:00:00

abstract：:The projected structure of the vanadate-induced crystalline aggregates of Ca2+-ATPase molecules in isolated sarcoplasmic reticulum membranes has been determined. The molecules form tubular crystals with an oblique surface lattice having cell dimensions a = 65.9 A, b = 114.4 A and gamma = 77.9 degrees. The space group ...

journal_title：Journal of molecular biology

authors： Taylor K,Dux L,Martonosi A

更新日期：1984-03-25 00:00:00

abstract：:A method that relates molecular structure to the forces that maintain it and to its X-ray diffraction pattern is described and applied to muscle. In a computer model, the potential energy of the movable components (here the myosin heads) is minimized by letting them move down the steepest gradient in three dimensions ...

journal_title：Journal of molecular biology

authors： Poulsen FR,Lowy J

更新日期：1984-03-25 00:00:00

abstract：:Crystalline complexes of tyrosyl-tRNA synthetase from Bacillus stearothermophilus were prepared with adenosine, AMP, ATP and PPi, all in the presence of tyrosinol, which binds strongly to the tyrosine binding site but cannot be adenylated by ATP. The hydrolysis of ATP in the presence of crystalline tyrosyl-tRNA synthe...

journal_title：Journal of molecular biology

authors： Monteilhet C,Blow DM,Brick P

更新日期：1984-03-15 00:00:00

abstract：:In the presence of AMP-PCP (beta, gamma-methyleneadenosine 5'-triphosphate), a non-hydrolyzable analog of ATP, negative stain images of increased morphological detail indicate that the dynein arm, attached to ciliary doublet microtubules, is composed of subunits including a cape, an elongated body and a head. The arra...

journal_title：Journal of molecular biology

authors： Avolio J,Lebduska S,Satir P

更新日期：1984-03-05 00:00:00

abstract：:The genes for alpha-fetoprotein and albumin arose by duplication of an ancestral gene that contained three genetic domains. These domains were generated by the triplication of a primordial genetic domain composed of five exons or subdomains. That the primordial domain itself arose by amplification of a simpler sequenc...

journal_title：Journal of molecular biology

authors： Alexander F,Young PR,Tilghman SM

更新日期：1984-02-25 00:00:00

abstract：:The transcription of the beta-globin genes in mouse erythroleukemia cells has been examined by hybridizing labeled RNA obtained from isolated nuclei after chain elongation in the presence of [alpha-32P]UTP. There is induction of at least 30-fold of beta maj globin transcription after cells are treated with either dime...

journal_title：Journal of molecular biology

authors： Salditt-Georgieff M,Sheffery M,Krauter K,Darnell JE Jr,Rifkind R,Marks PA

更新日期：1984-02-05 00:00:00

abstract：:The conformation of NAD+ in the sheep liver sorbitol dehydrogenase-NAD+ binary complex has been investigated using time-dependent proton-proton transferred nuclear Overhauser enhancement measurements to determine interproton distance ratios and distances between bound NAD+ protons. The conformation about both the aden...

journal_title：Journal of molecular biology

authors： Gronenborn AM,Clore GM,Jeffery J

更新日期：1984-02-05 00:00:00

abstract：:The discoidin I genes of Dictyostelium form a small, co-ordinately regulated multigene family. We have sequenced and compared the upstream regions of the DiscI-alpha, -beta and -gamma genes. For the most part the upstream regions of the three genes are non-homologous. The upstream sequences of the beta and gamma genes...

journal_title：Journal of molecular biology

authors： Poole SJ,Firtel RA

更新日期：1984-01-15 00:00:00

abstract：:The promoter and operator sequences of the Tn10-encoded tetracycline resistance operon are determined in vitro by transcription studies of purified DNA restriction fragments, protection of guanosine from methylation by dimethylsulphate, and DNase I footprinting employing the purified TET repressor protein. In vitro tr...

journal_title：Journal of molecular biology

authors： Hillen W,Schollmeier K,Gatz C

更新日期：1984-01-15 00:00:00

abstract：:We have investigated the structure of complexes formed between a series of poly(A)n (n = 30 to 480) and HD40 (helix-destabilizing protein, molecular weight of 40,000), the major protein component of 30 S heterogeneous nuclear ribonucleoprotein particles (hnRNP) from the brine shrimp Artemia salina. Protein HD40 is sim...

journal_title：Journal of molecular biology

authors： Thomas JO,Glowacka SK,Szer W

更新日期：1983-12-25 00:00:00

abstract：:The structure and activities of the recombination-promoting P22 Erf protein were examined in vitro. Treatment of the protein with elastase produces a stable amino-terminal fragment, consisting of amino acid residues 1 to (approximately) 136. We have purified this fragment, designated fragment B, to apparent homogeneit...

journal_title：Journal of molecular biology

authors： Poteete AR,Sauer RT,Hendrix RW

更新日期：1983-12-25 00:00:00

abstract：:Neutron-scattering in combination with quasi-elastic light-scattering and electron microscopy was used to derive a model for the capsid structure of the Top a-t component of alfalfa mosaic virus (AMV-Ta-t). In the electron microscope, AMV-Ta-t appears as an irregular ellipsoidal particle with apparent dimensions 275 (...

journal_title：Journal of molecular biology

authors： Cusack S,Oostergetel GT,Krijgsman PC,Mellema JE

更新日期：1983-12-05 00:00:00

abstract：:The structure of tomato bushy stunt virus has been determined crystallographically to 2.9 A resolution. Details are presented of both the molecular structure and the methods by which it has been solved. The icosahedrally symmetric viral shell is composed of 180 protein subunits (Mr 43,000), with three similar but dist...

journal_title：Journal of molecular biology

authors： Olson AJ,Bricogne G,Harrison SC

更新日期：1983-11-25 00:00:00

abstract：:In common with many other eukaryotic species, the genomes of species in the genus Allium contain a high proportion of repeated DNA sequences, which may be implicated in the considerable differences in genome size that are seen between even very closely related species. The gross organization of repetitive sequences wi...

journal_title：Journal of molecular biology

authors： Evans IJ,James AM,Barnes SR

更新日期：1983-11-15 00:00:00

abstract：:With a view to obtaining a more complete view of the composition and structure of the thick filaments of vertebrate skeletal muscle, we have isolated and characterized two new myofibrillar components, H-protein and X-protein. These were purified by hydroxyapatite column chromatography of an impure C-protein preparatio...

journal_title：Journal of molecular biology

authors： Starr R,Offer G

更新日期：1983-11-05 00:00:00

abstract：:Trichorzianine A 1 is one of the main components of a mixture of related antibiotic peptides (trichorzianines) produced by the fungus Trichoderma harzianum. Good crystals were obtained and allowed X-ray diffraction up to 0.8 A resolution. The space group is orthorhombic, C222(1), Z = 8, a = 64.8 (1) A, b = 9.33 (3) A,...

journal_title：Journal of molecular biology

authors： Bachet B,Brassy C,Morize I,Surcouf E,Mornon JP,Bodo B,Rebuffat S

更新日期：1983-11-05 00:00:00

abstract：:Six R sequences have been described as members of a new family of dispersed repetitive DNA in the mouse genome (Gebhard et al., 1982). Several sequenced regions were extended in the 5' direction (R1, R2 and R6) and two new sequences were determined (R7 and R8). On the basis of our sequence and blot hybridization data ...

journal_title：Journal of molecular biology

authors： Gebhard W,Zachau HG

更新日期：1983-10-25 00:00:00

abstract：:We report the X-ray structure analysis and refinement at 1.9 A resolution of calf gamma-II crystallin, a lens-specific protein. The sequence of Croft (1972) has been modified to give a polypeptide chain of 174 residues (cf. 165). The protein has a symmetrical, hierarchical structure of two globular domains each compri...

journal_title：Journal of molecular biology

authors： Wistow G,Turnell B,Summers L,Slingsby C,Moss D,Miller L,Lindley P,Blundell T

更新日期：1983-10-15 00:00:00

abstract：:A phage-neutralizing rabbit antiserum collected after immunization with tail-fiberless bacteriophage T4 particles was adsorbed with complete T4 phage. The resulting adsorbed serum inhibited tail fiber attachment in vitro. To identify the antigens against which this inhibitory activity was directed, blocking experiment...

journal_title：Journal of molecular biology

authors： Urig MA,Brown SM,Tedesco P,Wood WB

更新日期：1983-09-15 00:00:00

abstract：:Low dose electron diffraction and imaging techniques have been applied to the study of the crystalline structure of gp32*I, a DNA helix destabilizing protein derived from bacteriophage T4 gene 32 protein. A quantitative analysis of intensities from electron diffraction patterns from tilted, multilayered gp32*I crystal...

journal_title：Journal of molecular biology

authors： Cohen HA,Chiu W,Hosoda J

更新日期：1983-09-05 00:00:00

abstract：:The helical coiled-coil region of the myosin rod in the nematode Caenorhabditis elegans is a repetitive sequence 1094 amino acids long which contains 39 repeats of a 28-residue pattern. The repeats are extremely significant when compared with the statistical distributions expected, first for random sequences, and then...

journal_title：Journal of molecular biology

authors： McLachlan AD

更新日期：1983-09-05 00:00:00

abstract：:The 68C locus of the Drosophila melanogaster polytene chromosomes contains the structural genes for three glue polypeptides (sgs-3, sgs-7 and sgs-8) synthesized in the larval salivary glands during the third larval instar. When the messenger RNAs for the glue polypeptides are being synthesized, the locus is puffed; th...

journal_title：Journal of molecular biology

authors： Garfinkel MD,Pruitt RE,Meyerowitz EM

更新日期：1983-08-25 00:00:00

abstract：:The dielectric constants of sickle cell hemoglobin were determined before and after gelation. The dielectric properties of oxy and deoxy sickle cell hemoglobin in solution are nearly identical to those of oxy and deoxy hemoglobin A. Only in the gel state did deoxy sickle cell hemoglobin display dielectric behavior dif...

journal_title：Journal of molecular biology

authors： Delalic Z,Takashima S,Adachi K,Asakura T

更新日期：1983-08-15 00:00:00

abstract：:The motion of the atoms in the small protein bovine pancreatic trypsin inhibitor has been simulated for about 60 picoseconds using two different potential energy functions. In one known as HHL the hydrogen bond is purely electrostatic, in the other, known as L79, the hydrogen bond is a directional O . . . H interactio...

journal_title：Journal of molecular biology

authors： Levitt M

更新日期：1983-08-15 00:00:00

abstract：:The pfkB gene of Escherichia coli is known to specify a minor phosphofructokinase, Pfk-2, in the wild-type strain; the pfkB1 mutation causes a 25-fold increase in the amount of Pfk-2 so that it adequately substitutes for mutational loss of the major phosphofructokinase, Pfk-1 (specified by pfkA); and another closely l...

journal_title：Journal of molecular biology

authors： Daldal F

更新日期：1983-08-05 00:00:00

abstract：:The effect of divalent cations on the self-association of high molecular weight subfragment-2 (long S-2) and low molecular weight subfragment-2 (short S-2) of rabbit skeletal muscle myosin has been investigated. In the presence of millimolar concentrations of Ca2+ or Mg2+ long S-2 associates at neutral pH to form orde...

journal_title：Journal of molecular biology

authors： Ueno H,Rodgers ME,Harrington WF

更新日期：1983-08-05 00:00:00

abstract：:Alternating (dC-dG)n regions in DNA restriction fragments and recombinant plasmids were methylated at the 5 position of the cytosine residues by the HhaI methylase. Methylation lowers the concentration of NaCl or MgCl2 necessary to cause the B-Z conformational transition in these sequences. Ionic strengths higher than...

journal_title：Journal of molecular biology

authors： Klysik J,Stirdivant SM,Singleton CK,Zacharias W,Wells RD

更新日期：1983-07-25 00:00:00

abstract：:Electron microscopy and image processing of negatively stained crystalline sheets induced from Acanthamoeba actin have been used to yield a three-dimensional reconstruction of the actin molecule, including data to a maximum resolution of 15 A. This model shows actin to be an asymmetric, wedge-shaped molecule. A three-...

journal_title：Journal of molecular biology

authors： Smith PR,Fowler WE,Pollard TD,Aebi U

更新日期：1983-07-05 00:00:00

abstract：:We have studied in reconstitution experiments the conditions under which peptides derived from histones H1 and H5 are bound in chromatin and to what extent they are involved in the organization of chromatin fibers. The fragments of rat liver histone H1 (rH1) and chicken erythrocytes H1 (cH1) and H5 (cH5) used were the...

journal_title：Journal of molecular biology

authors： Thoma F,Losa R,Koller T

更新日期：1983-07-05 00:00:00

abstract：:Upon superinfection of immune (lysogenic) cells with bacteriophage Mu, a form of Mu DNA accumulates that sediments about twice as fast as the linear phage DNA marker in neutral sucrose gradients. This form is also detected upon infection of sensitive cells with Mu. We have purified it and examined its physical nature....

journal_title：Journal of molecular biology

authors： Harshey RM,Bukhari AI

更新日期：1983-06-25 00:00:00